Appendix 3 Laboratory Sample Container, Preservation, and Hold Times for Fresh Water Biological Sampling

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TYPE OF ANALYSIS SIZE TYPE PRESERVATION HOLD TIME

BACTERIOLOGICAL

Total Coliform 250 mL P, Ster keep cool, 4°C 48 h

Fecal Coliforms 250 mL P, Ster keep cool, 4°C 48 h

Fecal Streptococci 250 mL P, Ster keep cool, 4°C 48 h

E. coli 250 mL P, Ster keep cool, 4°C 48 h

Enterococci 250 mL P, Ster keep cool, 4°C 48 h

TAXONOMIC

Phytoplankton 1 L P 1-3 mL Lugol's 6 mo

Periphyton 250 mL P 1 mL Lugol's 6 mo

Zooplankton 250 mL P 70% ethanol 6 mo

Benthic invertebrates 500 mL P,W 70% ethanol 6 mo

LEGEND

P = plastic

Ster = sterile

W = wide mouth


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